ICC/IF analysis of COS7 cells transiently transfected with CD32a plasmid using GTX84509 CD32a antibody [16D6].
![IHC-P analysis of human bladder tissue using GTX84509 CD32a antibody [16D6]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX84509/GTX84509_2813_IHC-P_w_23061420_412.webp "IHC-P analysis of human bladder tissue using GTX84509 CD32a antibody [16D6]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.")
![IHC-P analysis of human colon tissue using GTX84509 CD32a antibody [16D6]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX84509/GTX84509_2814_IHC-P_w_23061420_662.webp "IHC-P analysis of human colon tissue using GTX84509 CD32a antibody [16D6]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.")
![IHC-P analysis of human endometrium tissue using GTX84509 CD32a antibody [16D6]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX84509/GTX84509_2815_IHC-P_w_23061420_862.webp "IHC-P analysis of human endometrium tissue using GTX84509 CD32a antibody [16D6]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.")
![IHC-P analysis of human ovary tissue using GTX84509 CD32a antibody [16D6]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX84509/GTX84509_2816_IHC-P_w_23061420_965.webp "IHC-P analysis of human ovary tissue using GTX84509 CD32a antibody [16D6]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.")
![IHC-P analysis of human pancreas tissue using GTX84509 CD32a antibody [16D6]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX84509/GTX84509_2817_IHC-P_w_23061420_928.webp "IHC-P analysis of human pancreas tissue using GTX84509 CD32a antibody [16D6]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.")
![IHC-P analysis of human prostate tissue using GTX84509 CD32a antibody [16D6]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX84509/GTX84509_2818_IHC-P_w_23061420_571.webp "IHC-P analysis of human prostate tissue using GTX84509 CD32a antibody [16D6]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.")
![IP analysis of DDDDK tagged CD32a overexpressed HEK293T lysate using GTX84509 CD32a antibody [16D6].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody. IP reaction : 2microg antibody / 500microl cell lysate](https://www.genetex.com/upload/website/prouct_img/normal/GTX84509/GTX84509_3338_IP_w_23061420_408.webp "IP analysis of DDDDK tagged CD32a overexpressed HEK293T lysate using GTX84509 CD32a antibody [16D6].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody. IP reaction : 2microg antibody / 500microl cell lysate")
![WB analysis of K562 cell lysate using GTX84509 CD32a antibody [16D6]. Loading : 10 ug per lane Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX84509/GTX84509_4006_WB_w_23061420_407.webp "WB analysis of K562 cell lysate using GTX84509 CD32a antibody [16D6]. Loading : 10 ug per lane Dilution : 1:200")
![FACS analysis of HEK293T cells transfected with either CD32a plasmid(Red) or empty vector control plasmid(Blue) using GTX84509 CD32a antibody [16D6].](https://www.genetex.com/upload/website/prouct_img/normal/GTX84509/GTX84509_439_FACS_w_23061420_740.webp "FACS analysis of HEK293T cells transfected with either CD32a plasmid(Red) or empty vector control plasmid(Blue) using GTX84509 CD32a antibody [16D6].")
ICC/IF analysis of COS7 cells transiently transfected with CD32a plasmid using GTX84509 CD32a antibody [16D6].
CD32a antibody [16D6]
GTX84509
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetFCGR2A
Overview
- SupplierGeneTex
- Product NameCD32a antibody [16D6] - Overexpression Validated
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000. ICC/IF: 1:100. IHC-P: 1:50. FACS: 1:100. IP: 2ug/500ul. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID16D6
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID2212
- Target nameFCGR2A
- Target descriptionFc fragment of IgG receptor IIa
- Target synonymsCD32; CD32A; CDw32; Fc fragment of IgG, low affinity IIa, receptor (CD32); Fc gamma receptor RIIa3; FCG2; fc-gamma-RIIa; FcGR; FCGR2; FCGR2A1; fcRII-a; IGFR2; igG Fc receptor II-a; Immunoglobulin G Fc receptor II; low affinity immunoglobulin gamma Fc region receptor II-a
- HostMouse
- IsotypeIgG1
- Protein IDP12318
- Protein NameLow affinity immunoglobulin gamma Fc region receptor II-a
- Scientific DescriptionBinds to the Fc region of immunoglobulins gamma. Low affinity receptor. By binding to IgG it initiates cellular responses against pathogens and soluble antigens.
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203