![IHC-P analysis of human breast tissue using GTX84508 CD32a antibody [20D1]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. IHC-P analysis of human breast tissue using GTX84508 CD32a antibody [20D1]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX84508/GTX84508_2807_IHC-P_w_23061420_743.webp)
IHC-P analysis of human breast tissue using GTX84508 CD32a antibody [20D1]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.
CD32a antibody [20D1]
GTX84508
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetFCGR2A
Overview
- SupplierGeneTex
- Product NameCD32a antibody [20D1] - Overexpression Validated
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500. IHC-P: 1:50. IP: 2ug/500ul. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID20D1
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID2212
- Target nameFCGR2A
- Target descriptionFc gamma receptor IIa
- Target synonymsCD32, CD32A, CDw32, FCG2, FCGR2, FCGR2A1, FcGR, FcgammaRIIa, IGFR2, low affinity immunoglobulin gamma Fc region receptor II-a, Fc fragment of IgG receptor IIa, Fc fragment of IgG, low affinity IIa, receptor (CD32), Fc gamma receptor RIIa3, Immunoglobulin G Fc receptor II, fc-gamma-RIIa, fcRII-a, igG Fc receptor II-a
- HostMouse
- IsotypeIgG1
- Protein IDP12318
- Protein NameLow affinity immunoglobulin gamma Fc region receptor II-a
- Scientific DescriptionBinds to the Fc region of immunoglobulins gamma. Low affinity receptor. By binding to IgG it initiates cellular responses against pathogens and soluble antigens.
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
![IHC-P analysis of human colon tissue using GTX84508 CD32a antibody [20D1]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. IHC-P analysis of human colon tissue using GTX84508 CD32a antibody [20D1]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX84508/GTX84508_2808_IHC-P_w_23061420_685.webp)
![IHC-P analysis of human endometrium tissue using GTX84508 CD32a antibody [20D1]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. IHC-P analysis of human endometrium tissue using GTX84508 CD32a antibody [20D1]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX84508/GTX84508_2809_IHC-P_w_23061420_177.webp)
![IHC-P analysis of human kidney tissue using GTX84508 CD32a antibody [20D1]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. IHC-P analysis of human kidney tissue using GTX84508 CD32a antibody [20D1]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX84508/GTX84508_2810_IHC-P_w_23061420_112.webp)
![IHC-P analysis of human pancreas tissue using GTX84508 CD32a antibody [20D1]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. IHC-P analysis of human pancreas tissue using GTX84508 CD32a antibody [20D1]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX84508/GTX84508_2811_IHC-P_w_23061420_921.webp)
![IHC-P analysis of human prostate tissue using GTX84508 CD32a antibody [20D1]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. IHC-P analysis of human prostate tissue using GTX84508 CD32a antibody [20D1]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX84508/GTX84508_2812_IHC-P_w_23061420_297.webp)
![IP analysis of DDDDK tagged CD32a overexpressed HEK293T lysate using GTX84508 CD32a antibody [20D1].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody. IP reaction : 2microg antibody / 500microl cell lysate IP analysis of DDDDK tagged CD32a overexpressed HEK293T lysate using GTX84508 CD32a antibody [20D1].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody. IP reaction : 2microg antibody / 500microl cell lysate](https://www.genetex.com/upload/website/prouct_img/normal/GTX84508/GTX84508_3337_IP_w_23061420_894.webp)
![WB analysis of HEK293T cells transfected with CD32a plasmid (Right) or empty vector (Left) for 48 hrs using GTX84508 CD32a antibody [20D1]. Loading : 5 ug per lane WB analysis of HEK293T cells transfected with CD32a plasmid (Right) or empty vector (Left) for 48 hrs using GTX84508 CD32a antibody [20D1]. Loading : 5 ug per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX84508/GTX84508_4433_WB_w_23061420_778.webp)
![WB analysis of various cell lines using GTX84508 CD32a antibody [20D1]. Loading : 35 ug per lane WB analysis of various cell lines using GTX84508 CD32a antibody [20D1]. Loading : 35 ug per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX84508/GTX84508_4434_WB_w_23061420_114.webp)

![ICC/IF analysis of COS7 cells transiently transfected with CD32a plasmid using GTX84509 CD32a antibody [16D6].](https://www.genetex.com/upload/website/prouct_img/normal/GTX84509/GTX84509_1116_ICCIF_w_23061420_227.webp)
![ICC/IF analysis of COS7 cells transiently transfected with CD32a plasmid using GTX84511 CD32a antibody [13D7].](https://www.genetex.com/upload/website/prouct_img/normal/GTX84511/GTX84511_1117_ICCIF_w_23061420_953.webp)
![ICC/IF analysis of COS7 cells transiently transfected with CD32a plasmid using GTX84513 CD32a antibody [13G9].](https://www.genetex.com/upload/website/prouct_img/normal/GTX84513/GTX84513_1118_ICCIF_w_23061420_196.webp)
![ICC/IF analysis of COS7 cells transiently transfected with CD32a plasmid using GTX84518 CD32a antibody [16E7].](https://www.genetex.com/upload/website/prouct_img/normal/GTX84518/GTX84518_1119_ICCIF_w_23061420_427.webp)

