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Immunofluorescence analysis of Raw264.7 cells using ENTPD1/CD39 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of Raw264.7 cells using ENTPD1/CD39 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of Raw264.7 cells using ENTPD1/CD39 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

CD39 Polyclonal Antibody

E-AB-63756
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    CD39 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    The protein encoded by this gene is a plasma membrane protein that hydrolyzes extracellular ATP and ADP to AMP. Inhibition of this proteins activity may confer anticancer benefits. Several transcript variants encoding different isoforms have been found for this gene.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD39/ENTPD1 using anti-CD39/ENTPD1 antibody (A03196-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD39/ENTPD1 antigen affinity purified polyclonal antibody (Catalog # A03196-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD39/ENTPD1 at approximately 80 kDa. The expected band size for CD39/ENTPD1 is at 80 kDa.
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Lane 1: Human Spleen; Lane 2: Mouse Placenta; Lane 3: Mouse Spleen; Probed CD39 (3G4) Monoclonal Antibody (bsm-54043R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
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FACS analysis of human peripheral blood using GTX00533 CD39 antibody [TU66].
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