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ProductsBack/CD4 antibody [W3/25] (FITC)
Staining of rat splenocytes with Mouse anti Rat CD4 (GTX76316)
Staining of rat splenocytes with Mouse anti Rat CD4 (GTX76316)
Staining of rat splenocytes with Mouse anti Rat CD4 (GTX76316)

CD4 antibody [W3/25] (FITC)

GTX76317
GeneTex
ApplicationsFlow Cytometry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityRat
TargetCd4
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Overview

  • Supplier
    GeneTex
  • Product Name
    CD4 antibody [W3/25] (FITC)
  • Delivery Days Customer
    9
  • Application Supplier Note
    For FACS: Use 0.5 ug to label 10^6 cells. Protocols are available upon request. Optimal dilutions/concentrations should be determined by the end user.
  • Applications
    Flow Cytometry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    W3/25
  • Concentration
    0.1 mg/ml
  • Conjugate
    FITC
  • Gene ID24932
  • Target name
    Cd4
  • Target description
    Cd4 molecule
  • Target synonyms
    W3/25, p55, T-cell surface glycoprotein CD4, CD4 antigen, T-cell surface antigen T4/Leu-3, W3/25 antigen
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP05540
  • Protein Name
    T-cell surface glycoprotein CD4
  • Scientific Description
    MHC class II binding protein that may be a candidate gene for collagen-induced arthritis; human CD4 is a receptor required for HIV infection [RGD, Feb 2006]
  • Reactivity
    Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CD4 using anti-CD4 antibody (A00344-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse RAW264.7 whole cell lysates, Lane 2: mouse ANA-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD4 antigen affinity purified polyclonal antibody (Catalog # A00344-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD4 at approximately 52KD. The expected band size for CD4 is at 52KD.
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