![CD44 antibody [N1N2], N-term detects CD44 protein at cell membrane on human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian carcinoma. CD44 antibody [N1N2], N-term (GTX112893) diluted at 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min CD44 antibody [N1N2], N-term detects CD44 protein at cell membrane on human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian carcinoma. CD44 antibody [N1N2], N-term (GTX112893) diluted at 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min](https://www.genetex.com/upload/website/prouct_img/normal/GTX112893/GTX112893_40079_20141125_IHC_3_w_23060500_721.webp)
CD44 antibody [N1N2], N-term detects CD44 protein at cell membrane on human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian carcinoma. CD44 antibody [N1N2], N-term (GTX112893) diluted at 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
CD44 antibody [N1N2], N-term
GTX112893
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Rat
TargetCD44
Overview
- SupplierGeneTex
- Product NameCD44 antibody [N1N2], N-term
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.65 mg/ml
- ConjugateUnconjugated
- Gene ID960
- Target nameCD44
- Target descriptionCD44 molecule (IN blood group)
- Target synonymsCDW44, CSPG8, ECM-III, ECMR-III, H-CAM, HCELL, HUTCH-1, HUTCH-I, Hermes-1, LHR, MC56, MDU2, MDU3, MIC4, Pgp1, CD44 antigen, CD44 molecule (Indian blood group), GP90 lymphocyte homing/adhesion receptor, Hermes antigen, In(Lu) related-p80, Indian blood group antigen, cell surface glycoprotein CD44, chondroitin sulfate proteoglycan 8, epican, extracellular matrix receptor III, hematopoietic cell E- and L-selectin ligand, heparan sulfate proteoglycan, homing cell adhesion molecule, homing function and Indian blood group system, hyaluronate receptor, phagocyte glycoprotein 1, phagocytic glycoprotein 1, soluble CD44
- HostRabbit
- IsotypeIgG
- Protein IDP16070
- Protein NameCD44 antigen
- Scientific DescriptionThe protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis. [provided by RefSeq]
- ReactivityHuman, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Chao YH, Wu HP, Wu KH, et al. An increase in CD3+CD4+CD25+ regulatory T cells after administration of umbilical cord-derived mesenchymal stem cells during sepsis. PLoS One. 2014,9(10):e110338. doi: 10.1371/journal.pone.0110338Read this paper
![Wild-type (WT) and CD44 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD44 antibody [N1N2], N-term (GTX112893) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Wild-type (WT) and CD44 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD44 antibody [N1N2], N-term (GTX112893) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX112893/GTX112893_40079_20170112_WB_KO_watermark_w_23060500_218.webp)
![CD44 antibody [N1N2], N-term detects CD44 protein at cell membrane on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. CD44 antibody [N1N2], N-term (GTX112893) diluted at 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min CD44 antibody [N1N2], N-term detects CD44 protein at cell membrane on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. CD44 antibody [N1N2], N-term (GTX112893) diluted at 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min](https://www.genetex.com/upload/website/prouct_img/normal/GTX112893/GTX112893_40079_20141124_IHC_2_w_23060500_113.webp)
![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with CD44 antibody [N1N2], N-term (GTX112893) diluted at 1:500 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with CD44 antibody [N1N2], N-term (GTX112893) diluted at 1:500 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.](https://www.genetex.com/upload/website/prouct_img/normal/GTX112893/GTX112893_40079_20200131_WB_competitor_watermark_w_23060500_223.webp)




![FACS analysis of human peripheral blood lymphocytes using GTX03492 CD44 antibody [Hermes-3]. Acquisition of >10, 000 events was performed. Green : Primary antibody Blue : Isotype control Dilution : 1:50](https://www.genetex.com/upload/website/prouct_img/normal/GTX03492/GTX03492_20220217_FACS_w_23053123_714.webp)

![CD44 antibody [GT462] detects CD44 protein at cell membrane by immunohistochemical analysis (Autostainer Formulated). Sample: Paraffin-embedded human breast cancer. CD44 stained by CD44 antibody [GT462] (GTX628895) diluted at 1:200. Antigen Retrieval: EDTA buffer, 20 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX628895/GTX628895_41232_20201016_IHC-P_outsource_w_23061202_831.webp)