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ProductsBack/CD45.1 antibody [A20]

CD45.1 antibody [A20]

GTX01463
GeneTex
ApplicationsFlow Cytometry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
Product group Antibodies
ReactivityMouse
TargetPtprc
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Overview

  • Supplier
    GeneTex
  • Product Name
    CD45.1 antibody [A20]
  • Delivery Days Customer
    9
  • Application Supplier Note
    *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    A20
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID19264
  • Target name
    Ptprc
  • Target description
    protein tyrosine phosphatase receptor type C
  • Target synonyms
    B220, CD45R, Cd45, L-CA, Ly-5, Lyt-4, T200, loc, receptor-type tyrosine-protein phosphatase C, leukocyte common antigen, lymphocyte antigen 5, lymphocyte common antigen
  • Host
    Mouse
  • Isotype
    IgG2a
  • Reactivity
    Mouse
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CD45/Ptprc using anti-CD45/Ptprc antibody (A00555-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse spleen tissue lysates, Lane 2: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD45/Ptprc antigen affinity purified polyclonal antibody (Catalog # A00555-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD45/Ptprc at approximately 250 kDa. The expected band size for CD45/Ptprc is at 145 kDa.
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