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ProductsBack/CD48 Antibody (clone B333)

CD48 Antibody (clone B333)

LS-C170949
LifeSpan BioSciences
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoHistoChemistry
Product group Antibodies
TargetCD48
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CD48 Antibody (clone B333)
  • Delivery Days Customer
    23
  • Applications
    Flow Cytometry, ImmunoFluorescence, ImmunoHistoChemistry
  • Applications Supplier
    Flo, IF (5 - 20 µg/ml), IHC (5 - 20 µg/ml)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    B333
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID962
  • Target name
    CD48
  • Target description
    CD48 molecule
  • Target synonyms
    BCM1; BCM1 surface antigen; BLAST; BLAST1; B-lymphocyte activation marker BLAST-1; CD48 antigen; CD48 antigen (B-cell membrane protein); hCD48; leukocyte antigen MEM-102; mCD48; MEM-102; signaling lymphocytic activation molecule 2; SLAM family member 2; SLAMF2; TCT.1
  • Host
    Mouse
  • Isotype
    IgG2a
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD48 using anti-CD48 antibody (A03281-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: CEM whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD48 antigen affinity purified polyclonal antibody (Catalog # A03281-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD48 at approximately 39KD. The expected band size for CD48 is at 28KD.
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