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ProductsBack/CD63 Antibody / LAMP-3

CD63 Antibody / LAMP-3

ORB749339
Biorbyt
ApplicationsFlow Cytometry, ImmunoFluorescence
Product group Antibodies
ReactivityHuman, Mouse
TargetCD63
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Overview

  • Supplier
    Biorbyt
  • Product Name
    CD63 Antibody / LAMP-3
  • Delivery Days Customer
    10
  • Application Supplier Note
    Optimal dilution of the CD63 antibody should be determined by the researcher.
  • Applications
    Flow Cytometry, ImmunoFluorescence
  • Applications Supplier
    Flow cytometry: 5ul per test per one million cells in 0.1ml or 5ul per 100ul of whole blood,Immunofluorescence: 1:50-1:100 FACS, IF
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    MX-49.129.5
  • Conjugate
    Other Conjugate
  • Gene ID967
  • Target name
    CD63
  • Target description
    CD63 molecule
  • Target synonyms
    AD1, HOP-26, ME491, MLA1, OMA81H, Pltgp40, TSPAN30, CD63 antigen, AD1 antigen, CD63 antigen (melanoma 1 antigen), granulophysin, limp1, melanoma-associated antigen ME491, melanoma-associated antigen MLA1, ocular melanoma-associated antigen, tetraspanin-30, tspan-30
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP08962
  • Protein Name
    CD63 antigen
  • Scientific Description
    Mouse monoclonal antibody against CD63 conjugated to CF488
  • Reactivity
    Human, Mouse
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD63 using anti-CD63 antibody (PB9250). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MDA-MB-453 whole cell lysates, Lane 2: human U87 whole cell lysates, Lane 3: human HL-60 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD63 antigen affinity purified polyclonal antibody (Catalog # PB9250) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD63 at approximately 30-60 kDa. The expected band size for CD63 is at 26 kDa.
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