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Cross-reactivity assessment of CD63 Mouse Recombinant Antibody AE00244 (1ug/ml) on CDI’s Protein Array containing more than 19,000 full-length human proteins.
Cross-reactivity assessment of CD63 Mouse Recombinant Antibody AE00244 (1ug/ml) on CDI’s Protein Array containing more than 19,000 full-length human proteins.
Cross-reactivity assessment of CD63 Mouse Recombinant Antibody AE00244 (1ug/ml) on CDI’s Protein Array containing more than 19,000 full-length human proteins.

CD63 Recombinant Antibody AE00244

AE00244
Aeonian Biotech
TargetCD63
Product group Antibodies
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Overview

  • Supplier
    Aeonian Biotech
  • Product Name
    CD63 Recombinant Antibody AE00244
  • Delivery Days Customer
    9
  • Applications Supplier
    IHC, PA
  • Certification
    Research Use Only
  • Clonality
    Recombinant
  • Clone ID
    rMX-49.129.5
  • Conjugate
    Unconjugated
  • Gene ID967
  • Target name
    CD63
  • Target description
    CD63 molecule
  • Target synonyms
    AD1, HOP-26, ME491, MLA1, OMA81H, Pltgp40, TSPAN30, CD63 antigen, AD1 antigen, CD63 antigen (melanoma 1 antigen), granulophysin, limp1, melanoma-associated antigen ME491, melanoma-associated antigen MLA1, ocular melanoma-associated antigen, tetraspanin-30, tspan-30
  • Host
    Mouse
  • Isotype
    IgG1 kappa
  • Scientific Description
    CD63 Recombinant Antibody AE00244
  • Shelf life instruction
    Integrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
  • Reactivity Supplier
    Human
  • Storage Instruction
    2-8°C
  • UNSPSC
    12352203

Datasheet

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MSDS

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FAQ

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Figure 1. Western blot analysis of CD63 using anti-CD63 antibody (PB9250). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MDA-MB-453 whole cell lysates, Lane 2: human U87 whole cell lysates, Lane 3: human HL-60 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD63 antigen affinity purified polyclonal antibody (Catalog # PB9250) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD63 at approximately 30-60 kDa. The expected band size for CD63 is at 26 kDa.
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