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IHC-Fr analysis of mouse lymph node tissue using GTX41866 CD68 antibody [FA-11].
IHC-Fr analysis of mouse lymph node tissue using GTX41866 CD68 antibody [FA-11].
IHC-Fr analysis of mouse lymph node tissue using GTX41866 CD68 antibody [FA-11].

CD68 antibody [FA-11]

GTX41866
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetCd68
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Overview

  • Supplier
    GeneTex
  • Product Name
    CD68 antibody [FA-11]
  • Delivery Days Customer
    9
  • Application Supplier Note
    FCM: 1/50-1/100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    FA-11
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID12514
  • Target name
    Cd68
  • Target description
    CD68 antigen
  • Target synonyms
    Lamp4, Scard1, gp110, macrosialin
  • Host
    Rat
  • Isotype
    IgG2a
  • Protein IDP31996
  • Protein Name
    Macrosialin
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CD68 using anti-CD68 antibody (PA1518). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates, Lane 2: mouse spleen tissue lysates, Lane 3: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD68 antigen affinity purified polyclonal antibody (Catalog # PA1518) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD68 at approximately 90-100 kDa. The expected band size for CD68 is at 37 kDa.
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