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ProductsBack/CD72 Antibody

CD72 Antibody

LS-C411433
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetCD72
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CD72 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 40kDa, while the observed MW by Western blot was 44kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:1000 - 1:4000) The predicted MW is 40kDa, while the observed MW by Western blot was 44kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID971
  • Target name
    CD72
  • Target description
    CD72 molecule
  • Target synonyms
    CD72b, LYB2, B-cell differentiation antigen CD72, CD72 antigen, lyb-2
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD72 using anti-CD72 antibody (A09292-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A375 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD72 antigen affinity purified polyclonal antibody (Catalog # A09292-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD72 at approximately 45KD. The expected band size for CD72 is at 40KD.
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