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ProductsBack/Cd80 Rat Monoclonal Antibody [Clone ID: 1G10]
Immunofluorescent Staining with CD80 antibody [1G10] (FITC): P815 cells transfected with CD80 or non-transfected P815 control cells were stained with Rat anti-Mouse CD80 antibody (FITC). Large cells were then gated and analyzed on a FACScan (TM) flow cytometer (BDIS, San Jose, CA). Amount Used: < / = 3 ug/10e6 cells.
Immunofluorescent Staining with CD80 antibody [1G10] (FITC): P815 cells transfected with CD80 or non-transfected P815 control cells were stained with Rat anti-Mouse CD80 antibody (FITC). Large cells were then gated and analyzed on a FACScan (TM) flow cytometer (BDIS, San Jose, CA). Amount Used: &lt; / = 3 ug/10e6 cells.
Immunofluorescent Staining with CD80 antibody [1G10] (FITC): P815 cells transfected with CD80 or non-transfected P815 control cells were stained with Rat anti-Mouse CD80 antibody (FITC). Large cells were then gated and analyzed on a FACScan (TM) flow cytometer (BDIS, San Jose, CA). Amount Used: < / = 3 ug/10e6 cells.

Cd80 Rat Monoclonal Antibody [Clone ID: 1G10]

AM08052FC-N
OriGene
ApplicationsFlow Cytometry
Product group Antibodies
ReactivityMouse
TargetCd80
Price on request
500 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    Cd80 Rat Monoclonal Antibody [Clone ID: 1G10]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    1G10
  • Gene ID12519
  • Target name
    Cd80
  • Target description
    CD80 antigen
  • Target synonyms
    B71, Cd28l, Ly-53, Ly53, MIC17, TSA1, T-lymphocyte activation antigen CD80, B7 protein, activation B7-1 antigen
  • Host
    Rat
  • Isotype
    IgG2a
  • Protein IDQ00609
  • Protein Name
    T-lymphocyte activation antigen CD80
  • Scientific Description
    Cd80 rat monoclonal antibody, clone 1G10, FITC
  • Reactivity
    Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of Cd80 using anti-Cd80 antibody (A00196-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Daudi whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: mouse RAW264.7 whole cell lysates, Lane 4: mouse ANA-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cd80 antigen affinity purified polyclonal antibody (Catalog # A00196-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cd80 at approximately 70 kDa. The expected band size for Cd80 is at 33 kDa.
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