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WB analysis of various samples using GTX66709 CD9 antibody. Lane1 : THP-1 whole cell lysate(40ug) Lane2 : The Peripheral blood cells tissue lysate of Rat(30ug) Lane3 : The Peripheral blood cells tissue lysate of Mouse(30ug) Dilution : 1:500
WB analysis of various samples using GTX66709 CD9 antibody. Lane1 : THP-1 whole cell lysate(40ug) Lane2 : The Peripheral blood cells tissue lysate of Rat(30ug) Lane3 : The Peripheral blood cells tissue lysate of Mouse(30ug) Dilution : 1:500
WB analysis of various samples using GTX66709 CD9 antibody. Lane1 : THP-1 whole cell lysate(40ug) Lane2 : The Peripheral blood cells tissue lysate of Rat(30ug) Lane3 : The Peripheral blood cells tissue lysate of Mouse(30ug) Dilution : 1:500

CD9 antibody

GTX66709
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetCD9
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Overview

  • Supplier
    GeneTex
  • Product Name
    CD9 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID928
  • Target name
    CD9
  • Target description
    CD9 molecule
  • Target synonyms
    5H9 antigen; antigen CD9; BA-2/p24 antigen; BTCC-1; CD9 antigen; CD9 antigen (p24); cell growth-inhibiting gene 2 protein; DRAP-27; leukocyte antigen MIC3; MIC3; motility related protein-1; MRP-1; tetraspanin-29; TSPAN29; TSPAN-29
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP21926
  • Protein Name
    CD9 antigen
  • Scientific Description
    This gene encodes a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Tetraspanins are cell surface glycoproteins with four transmembrane domains that form multimeric complexes with other cell surface proteins. The encoded protein functions in many cellular processes including differentiation, adhesion, and signal transduction, and expression of this gene plays a critical role in the suppression of cancer cell motility and metastasis. [provided by RefSeq, Jan 2011]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

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  • Therapeutic effect of induced pluripotent stem cell -derived extracellular vesicles in an in vitro and in vivo osteoarthritis model.
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  • Human Olfactory Ensheathing Cell-derived Extracellular Cesicles: miRNA Profile and Neuroprotective Effect. Tu YK et al., 2021, Curr Neurovasc Res
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Figure 1. Western blot analysis of CD9 using anti-CD9 antibody (A01202-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HCCT tissue lysates, Lane 2: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD9 antigen affinity purified polyclonal antibody (Catalog # A01202-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD9 at approximately 22 kDa. The expected band size for CD9 is at 25 kDa.
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