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ProductsBack/CD9 antibody [MEM-61] (APC)

CD9 antibody [MEM-61] (APC)

GTX79930
GeneTex
ApplicationsFlow Cytometry
Product group Antibodies
ReactivityHuman
TargetCD9
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Overview

  • Supplier
    GeneTex
  • Product Name
    CD9 antibody [MEM-61] (APC)
  • Delivery Days Customer
    9
  • Application Supplier Note
    FACS: 10 microl reagent / 100 microl of whole blood or 106 cells in a suspension. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    MEM-61
  • Conjugate
    APC (Allophycocyanin)
  • Gene ID928
  • Target name
    CD9
  • Target description
    CD9 molecule
  • Target synonyms
    BTCC-1, DRAP-27, MIC3, MRP-1, TSPAN-29, TSPAN29, CD9 antigen, 5H9 antigen, BA-2/p24 antigen, CD9 antigen (p24), antigen CD9, cell growth-inhibiting gene 2 protein, leukocyte antigen MIC3, motility related protein-1, tetraspanin-29
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP21926
  • Protein Name
    CD9 antigen
  • Scientific Description
    This gene encodes a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Tetraspanins are cell surface glycoproteins with four transmembrane domains that form multimeric complexes with other cell surface proteins. The encoded protein functions in many cellular processes including differentiation, adhesion, and signal transduction, and expression of this gene plays a critical role in the suppression of cancer cell motility and metastasis. [provided by RefSeq, Jan 2011]
  • Reactivity
    Human
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

References

  • Gotanda K, Hirota T, Saito J, et al. Circulating intestine-derived exosomal miR-328 in plasma, a possible biomarker for estimating BCRP function in the human intestines. Sci Rep. 2016,6:32299. doi: 10.1038/srep32299
    Read this paper
  • Lafleur MA, Xu D, Hemler ME. Tetraspanin proteins regulate membrane type-1 matrix metalloproteinase-dependent pericellular proteolysis. Mol Biol Cell. 2009,20(7):2030-40.
    Read this paper

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Figure 1. Western blot analysis of CD9 using anti-CD9 antibody (A01202-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HCCT tissue lysates, Lane 2: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD9 antigen affinity purified polyclonal antibody (Catalog # A01202-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD9 at approximately 22 kDa. The expected band size for CD9 is at 25 kDa.
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