Bio-Connect

CD99 Polyclonal Antibody

RD252527A
Reddot Biotech
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetCD99
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request

Overview

  • Supplier
    Reddot Biotech
  • Product Name
    CD99 Polyclonal Antibody
  • Delivery Days Customer
    5
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.9 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID4267
  • Target name
    CD99
  • Target description
    CD99 molecule (Xg blood group)
  • Target synonyms
    antigen identified by monoclonal 12E7, Y homolog; antigen identified by monoclonal antibodies 12E7, F21 and O13; CD99 antigen; cell surface antigen 12E7; cell surface antigen HBA-71; cell surface antigen O13; E2 antigen; HBA71; MIC2; MIC2 (monoclonal antibody 12E7); MIC2X; MIC2Y; MSK5X; surface antigen MIC2; T-cell surface glycoprotein E2
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    CD99,also known as MIC2,is a heavily O-glycosylated transmembrane protein involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. CD99 is broadly distributed on many cell types,with particularly strong expression on human cortical thymocytes,Ewing,Aeos sarcoma cells and peripheral primitive neuroectodermal tumors . In normal cells,CD99 has been functionally implicated in cell adhesion,migration,apoptosis,differentiation,activation,and proliferation of lymphocytes and monocyte extravasation and transport of several transmembrane proteins . CD99 displays two surface isoforms generated by alternative splicing: a long 32 kDa and a short 28 kDa form .
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Related products

Product group Antibodies
Assay Genie
TargetCD99
  • SizePrice
Product group Antibodies
Biomatik
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetCD99
  • SizePrice
Anti-CD99 Antibody
Product group Antibodies
Antibodies.com
ApplicationsImmunoFluorescence, ELISA
  • SizePrice
Product group Antibodies
GeneTex
CD99 antibodyGTX113359
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetCD99
  • SizePrice
Product group Antibodies
MedChem Express
CD99 AntibodyHY-P80070
TargetCD99
  • SizePrice
Product group Antibodies
Cusabio
CD99 AntibodyCSB-PA010191
ApplicationsImmunoFluorescence, ELISA
TargetCD99
  • SizePrice
Figure 1. Western blot analysis of CD99 using anti-CD99 antibody (A01724-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates, Lane 2: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD99 antigen affinity purified polyclonal antibody (Catalog # A01724-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD99 at approximately 28 kDa. The expected band size for CD99 is at 28 kDa.
Product group Antibodies
Boster Bio
Anti-CD99 Antibody Picoband(r)A01724-2-CARRIER-FREE
ApplicationsWestern Blot
TargetCD99
  • SizePrice
Immunohistochemical staining of human ovarian cancer (mucinous carcinoma) shows moderate membranous positivity in tumor cells.
Product group Antibodies
Atlas Antibodies
ApplicationsImmunoHistoChemistry
TargetCD99
  • SizePrice
Product group Antibodies
RayBiotech
ApplicationsWestern Blot
TargetCD99
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
CD99 AntibodyLS-C831952
ApplicationsELISA, ImmunoHistoChemistry
TargetCD99
  • SizePrice