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WB analysis of extracts from 3T3 cells untreated (lane 1) or treated with Hydroxyurea (lane 2) using GTX50360 CDC25B (phospho Ser149) antibody.
WB analysis of extracts from 3T3 cells untreated (lane 1) or treated with Hydroxyurea (lane 2) using GTX50360 CDC25B (phospho Ser149) antibody.
WB analysis of extracts from 3T3 cells untreated (lane 1) or treated with Hydroxyurea (lane 2) using GTX50360 CDC25B (phospho Ser149) antibody.

CDC25B (phospho Ser149) antibody

GTX50360
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse
TargetCdc25b
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Overview

  • Supplier
    GeneTex
  • Product Name
    CDC25B (phospho Ser149) antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID12531
  • Target name
    Cdc25b
  • Target description
    cell division cycle 25B
  • Target synonyms
    M-phase inducer phosphatase 2, dual specificity phosphatase Cdc25B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP30306
  • Protein Name
    M-phase inducer phosphatase 2
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Cdc25b using anti-Cdc25b antibody (A01899). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cdc25b antigen affinity purified polyclonal antibody (Catalog # A01899) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cdc25b at approximately 65 kDa. The expected band size for Cdc25b is at 65 kDa.
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