More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/Phospho-Cdc25b (Ser149) Antibody

Phospho-Cdc25b (Ser149) Antibody

PACO24346
Assay Genie
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityMouse, Rat
TargetCdc25b
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Assay Genie
  • Product Name
    Phospho-Cdc25b (Ser149) Antibody
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID12531
  • Target name
    Cdc25b
  • Target description
    cell division cycle 25B
  • Target synonyms
    M-phase inducer phosphatase 2, dual specificity phosphatase Cdc25B
  • Host
    Rabbit
  • Protein IDQ9DBN8
  • Protein Name
    M-phase inducer phosphatase
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity
    Mouse, Rat
  • Reactivity Supplier
    Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

Datasheet

Download Datasheet

MSDS

Download MSDS

FAQ

Go to our frequently asked questions

Related products

Antigen: bs-8445P, 0.2ug/100ul \nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\nTMB staining;\nRead the data in MicroplateReader by 450
Product group Antibodies
Bioss
phospho-Cdc25B (Ser149) Polyclonal AntibodyBS-8445R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetCdc25b
  • SizePrice
WB analysis of extracts from HT-29 cells treated with PMA or calf intestinal phosphatase (CIP) using GTX50360 CDC25B (phospho Ser149) antibody.
Product group Antibodies
GeneTex
CDC25B (phospho Ser149) antibodyGTX50360
ApplicationsWestern Blot
ReactivityHuman, Mouse
TargetCdc25b
  • SizePrice
Western blot analysis of extracts from 3T3 cells untreated(lane 1) or treated with Hydroxyurea(lane 2) using Cdc25B(Phospho-Ser149) Antibody.
Product group Antibodies
Cusabio
Phospho-Cdc25b (Ser149) AntibodyCSB-PA905252
ApplicationsWestern Blot, ELISA
ReactivityMouse, Rat
TargetCdc25b
  • SizePrice
Figure 1. Western blot analysis of Cdc25b using anti-Cdc25b antibody (A01899). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cdc25b antigen affinity purified polyclonal antibody (Catalog # A01899) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cdc25b at approximately 65 kDa. The expected band size for Cdc25b is at 65 kDa.
Product group Antibodies
Boster Bio
Anti-Cdc25b Antibody Picoband(r)A01899-CARRIER-FREE
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityMouse, Rat
TargetCdc25b
  • SizePrice