Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-Cdc25B (Ser186) Polyclonal Antibody, Unconjugated (bs-5244R) at 1:500 overnight at 4°C, followed by a conjugated secondary for 90 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-Cdc25B (Ser186) Polyclonal Antibody, Unconjugated (bs-5244R) at 1:500 overnight at 4°C, followed by a conjugated secondary for 90 minutes and DAB staining.
Cdc25B (Ser186) Polyclonal Antibody
BS-5244R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCDC25B
Overview
- SupplierBioss Antibodies
- Product NameCdc25B (Ser186) Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierWB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID994
- Target nameCDC25B
- Target descriptioncell division cycle 25B
- Target synonymsCDC25 homolog B; dual specificity phosphatase Cdc25B; M-phase inducer phosphatase 2
- HostRabbit
- IsotypeIgG
- Protein IDP30305
- Protein NameM-phase inducer phosphatase 2
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C
- UNSPSC12352203