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Formalin-fixed and paraffin embedded human rectal carcinoma labeled with Anti-Phospho-cdc25C (Thr48) Polyclonal Antibody, Unconjugated (bs-3482R) at 1:200 followed by conjugation to the secondary antibody and DAB
Formalin-fixed and paraffin embedded human rectal carcinoma labeled with Anti-Phospho-cdc25C (Thr48) Polyclonal Antibody, Unconjugated (bs-3482R) at 1:200 followed by conjugation to the secondary antibody and DAB
Formalin-fixed and paraffin embedded human rectal carcinoma labeled with Anti-Phospho-cdc25C (Thr48) Polyclonal Antibody, Unconjugated (bs-3482R) at 1:200 followed by conjugation to the secondary antibody and DAB

Phospho-cdc25C (Thr48) Polyclonal Antibody

BS-3482R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCDC25C
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    cdc25C (Thr48) Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID995
  • Target name
    CDC25C
  • Target description
    cell division cycle 25C
  • Target synonyms
    CDC25; CDC25 homolog C; dual specificity phosphatase CDC25C; mitosis inducer CDC25; M-phase inducer phosphatase 3; phosphotyrosine phosphatase; PPP1R60; protein phosphatase 1, regulatory subunit 60
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP30307
  • Protein Name
    M-phase inducer phosphatase 3
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • The molecular mechanism of G2M cell cycle arrest induced by AFB1 in the jejunum. Yin H et al., 2016 Jun 14, Oncotarget
    Read more
  • Dietary NiCl2 causes G2/M cell cycle arrest in the broilers kidney. Guo H et al., 2015 Nov 3, Oncotarget
    Read more

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Figure 1. Western blot analysis of Cdc25C using anti-Cdc25C antibody (PB9756). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Ovary Tissue Lysate at 50ug, Lane 2: Rat Liver Tissue Lysate at 50ug, Lane 3: HELA Whole Cell Lysate at 40ug, Lane 4: SW620 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cdc25C antigen affinity purified polyclonal antibody (Catalog # PB9756) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cdc25C at approximately 60 kDa. The expected band size for Cdc25C is at 60 kDa.
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