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CDC73 / Parafibromin Antibody

LS-C813485
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetCDC73
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CDC73 / Parafibromin Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA (1:5000 - 1:20000), WB (1:500 - 1:2000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID79577
  • Target name
    CDC73
  • Target description
    cell division cycle 73
  • Target synonyms
    C1orf28; cell division cycle 73 Paf1/RNA polymerase II complex component-like protein; cell division cycle 73, Paf1/RNA polymerase II complex component, homolog; cell division cycle protein 73 homolog; Familial isolated hyperparathyroidism; FIHP; HPTJT; HRPT1; HRPT2; hyperparathyroidism 2 protein; HYX; Paf1/RNA polymerase II complex component; parafibromin
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HRPT2 using anti-HRPT2 antibody (PB9532). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Human Placenta Tissue Lysate at 50ug, Lane 2: HEPG2 Whole Cell Lysate at 40ug, Lane 3: 293T Whole Cell Lysate at 40ug, Lane 4: HELA Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HRPT2 antigen affinity purified polyclonal antibody (Catalog # PB9532) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HRPT2 at approximately 61 kDa. The expected band size for HRPT2 is at 61 kDa.
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