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Western Blot analysis of HepG2 cells using CdcA3 Polyclonal Antibody
Western Blot analysis of HepG2 cells using CdcA3 Polyclonal Antibody
Western Blot analysis of HepG2 cells using CdcA3 Polyclonal Antibody

CDCA3 Antibody

CSB-PA001515
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCDCA3
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Overview

  • Supplier
    Cusabio
  • Product Name
    CDCA3 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID83461
  • Target name
    CDCA3
  • Target description
    cell division cycle associated 3
  • Target synonyms
    GRCC8, TOME-1, TOME1, cell division cycle-associated protein 3, gene-rich cluster protein C8, trigger of mitotic entry protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ99618
  • Protein Name
    Cell division cycle-associated protein 3
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CDCA3 using anti-CDCA3 antibody (M10074). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: rat heart tissue lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse heart tissue lysates, Lane 7: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDCA3 antigen affinity purified monoclonal antibody (Catalog # M10074) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDCA3 at approximately 29 kDa. The expected band size for CDCA3 is at 29 kDa.
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