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CDCA3 Antibody (YA3176, Rabbit)

HY-P83431
MedChem Express
TargetCDCA3
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    CDCA3 Antibody (YA3176, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID83461
  • Target name
    CDCA3
  • Target description
    cell division cycle associated 3
  • Target synonyms
    GRCC8, TOME-1, TOME1, cell division cycle-associated protein 3, gene-rich cluster protein C8, trigger of mitotic entry protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    CDCA3 Antibody (YA3176) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA3176), targeting CDCA3, with a predicted molecular weight of 29 kDa (observed band size: 26 kDa). CDCA3 Antibody (YA3176) can be used for WB, ICC/IF experiment in human, mouse, rat background.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CDCA3 using anti-CDCA3 antibody (M10074). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: rat heart tissue lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse heart tissue lysates, Lane 7: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDCA3 antigen affinity purified monoclonal antibody (Catalog # M10074) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDCA3 at approximately 29 kDa. The expected band size for CDCA3 is at 29 kDa.
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