Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CDK2 (phospho Tyr15) antibody [HL1474] (GTX636948) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![Untreated (–) and treated (+) PC-12 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CDK2 (phospho Tyr15) antibody [HL1474] (GTX636948) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636948/GTX636948_T-44662_20230714_WB_R_treatment_CIP_23071822_308.webp "Untreated (–) and treated (+) PC-12 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CDK2 (phospho Tyr15) antibody [HL1474] (GTX636948) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CDK2 (phospho Tyr15) antibody [HL1474] (GTX636948) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636948/GTX636948_T-44662_20230714_WB_treatment_CIP_23071822_239.webp "Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CDK2 (phospho Tyr15) antibody [HL1474] (GTX636948) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Specificity test was performed by coating the plate with CDK1/2/3/5 peptide and CDK1/2/3/5 (phospho Tyr15) peptide (15.63 ng/mL). Coated peptides were probed with CDK2 (phospho Tyr15) antibody [HL1474] (GTX636948) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636948/GTX636948_44711_20230721_ELISA_Indirect_Multiple_Bar_23072519_460.webp "Specificity test was performed by coating the plate with CDK1/2/3/5 peptide and CDK1/2/3/5 (phospho Tyr15) peptide (15.63 ng/mL). Coated peptides were probed with CDK2 (phospho Tyr15) antibody [HL1474] (GTX636948) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.")
![Drosophila tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with CDK2 (phospho Tyr15) antibody [HL1474] (GTX636948) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636948/GTX636948_44711_20230721_WB_Drosophila_brain_23073119_414.webp "Drosophila tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with CDK2 (phospho Tyr15) antibody [HL1474] (GTX636948) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![CDK2 (phospho Tyr15) antibody [HL1474] detects CDK2 (phospho Tyr15) protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. CDK2 (phospho Tyr15) stained by CDK2 (phospho Tyr15) antibody [HL1474] (GTX636948) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX636948/GTX636948_44711_20240401_IHC-P_M_24041019_395.webp "CDK2 (phospho Tyr15) antibody [HL1474] detects CDK2 (phospho Tyr15) protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. CDK2 (phospho Tyr15) stained by CDK2 (phospho Tyr15) antibody [HL1474] (GTX636948) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CDK2 (phospho Tyr15) antibody [HL1474] (GTX636948) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CDK2 (phospho Tyr15) antibody [HL1474]
GTX636948
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityDrosophila, Human, Mouse, Rat
TargetCDK2
Overview
- SupplierGeneTex
- Product NameCDK2 (phospho Tyr15) antibody [HL1474]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL1474
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID1017
- Target nameCDK2
- Target descriptioncyclin dependent kinase 2
- Target synonymsCDKN2, p33(CDK2), cyclin-dependent kinase 2, cdc2-related protein kinase, cell division protein kinase 2, p33 protein kinase
- HostRabbit
- IsotypeIgG
- Protein IDP24941
- Protein NameCyclin-dependent kinase 2
- Scientific DescriptionThis gene encodes a member of a family of serine/threonine protein kinases that participate in cell cycle regulation. The encoded protein is the catalytic subunit of the cyclin-dependent protein kinase complex, which regulates progression through the cell cycle. Activity of this protein is especially critical during the G1 to S phase transition. This protein associates with and regulated by other subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A), and p27Kip1 (CDKN1B). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]
- ReactivityDrosophila, Human, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
Datasheet
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