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CDK4 Antibody (Preservative Free)

LS-C815551
LifeSpan BioSciences
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCDK4
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CDK4 Antibody (Preservative Free)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ICC (1:300 - 1:10000), IF (1:300 - 1:10000), IHC-P (1:500 - 1:2000), IP, WB (1:500 - 1:2000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID1019
  • Target name
    CDK4
  • Target description
    cyclin dependent kinase 4
  • Target synonyms
    cell division protein kinase 4; CMM3; cyclin-dependent kinase 4; PSK-J3
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CDK4 using anti-CDK4 antibody (PA1428-1, Left) and anti-CDK4 antibody (PB9535, Right). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: rat PC-12 whole cell lysates, Lane 4: mosue NIH/3T3 whole cell lysates, Lane 5: mosue RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDK4 antigen affinity purified polyclonal antibody (Catalog # PA1428-1) and rabbit anti-CDK4 antigen affinity purified polyclonal antibody (Catalog # PB9535)at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002)?with Tanon 5200 system. A specific band was detected for CDK4 at approximately 34 kDa. The expected band size for CDK4 is at 34 kDa.
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