Paraformaldehyde-fixed, paraffin embedded rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with CDK4 Polyclonal Antibody, Unconjugated (bs-0633R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with CDK4 Polyclonal Antibody, Unconjugated (bs-0633R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.
CDK4 Polyclonal Antibody
BS-0633R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Human, Mouse, Porcine, Rat, Sheep
TargetCDK4
Overview
- SupplierBioss Antibodies
- Product NameCDK4 Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierWB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID1019
- Target nameCDK4
- Target descriptioncyclin dependent kinase 4
- Target synonymscell division protein kinase 4; CMM3; cyclin-dependent kinase 4; PSK-J3
- HostRabbit
- IsotypeIgG
- Protein IDP11802
- Protein NameCyclin-dependent kinase 4
- ReactivityBovine, Human, Mouse, Porcine, Rat, Sheep
- Storage Instruction-20°C
- UNSPSC12352203