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Cdk6 Recombinant Antibody

CAC11869
Biomatik
ApplicationsImmunoPrecipitation, Western Blot, ELISA
Product group Antibodies
TargetCDK6
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Overview

  • Supplier
    Biomatik
  • Product Name
    CDK6 Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoPrecipitation, Western Blot, ELISA
  • Applications Supplier
    ELISA, WB, IP; Recommended dilution: WB:1:500-1:5000, IP:1:200-1:1000
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    8G3
  • Conjugate
    Unconjugated
  • Gene ID1021
  • Target name
    CDK6
  • Target description
    cyclin dependent kinase 6
  • Target synonyms
    cell division protein kinase 6; cyclin-dependent kinase 6; MCPH12; PLSTIRE; serine/threonine-protein kinase PLSTIRE
  • Isotype
    IgG
  • Protein IDQ00534
  • Protein Name
    Cyclin-dependent kinase 6
  • Scientific Description
    The Cdk6 Recombinant Antibody (Species: Human) has been validated for the following applications: ELISA, WB, IP.
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Cdk6 using anti-Cdk6 antibody (A00358). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cdk6 antigen affinity purified polyclonal antibody (Catalog # A00358) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cdk6 at approximately 37 kDa. The expected band size for Cdk6 is at 37 kDa.
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