More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/CDK6 Polyclonal Antibody
Paraformaldehyde-fixed, paraffin embedded human glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with CDK6 Polyclonal Antibody, Unconjugated (bs-0568R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with CDK6 Polyclonal Antibody, Unconjugated (bs-0568R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with CDK6 Polyclonal Antibody, Unconjugated (bs-0568R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.

CDK6 Polyclonal Antibody

BS-0568R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCDK6
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Bioss
  • Product Name
    CDK6 Polyclonal Antibody
  • Delivery Days Customer
    7
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID1021
  • Target name
    CDK6
  • Target description
    cyclin dependent kinase 6
  • Target synonyms
    MCPH12, PLSTIRE, cyclin-dependent kinase 6, cell division protein kinase 6, serine/threonine-protein kinase PLSTIRE
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ00534
  • Protein Name
    Cyclin-dependent kinase 6
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • He X, Zheng Y, Zhang Y, et al. Long non-coding RNA AK058003, as a precursor of miR-15a, interacts with HuR to inhibit the expression of γ-synuclein in hepatocellular carcinoma cells. Oncotarget. 2017,8(6):9451-9465. doi: 10.18632/oncotarget.14276
    Read this paper
  • Hu Z, Fan H, Lv G, et al. 5-Aminolevulinic acid-mediated sonodynamic therapy induces anti-tumor effects in malignant melanoma via p53-miR-34a-Sirt1 axis. J Dermatol Sci. 2015,79(2):155-62. doi: 10.1016/j.jdermsci.2015.04.010
    Read this paper
  • Zhang J, Wang L, Li B, et al. miR-145 downregulates the expression of cyclin-dependent kinase 6 in human cervical carcinoma cells. Exp Ther Med. 2014,8(2):591-594.
    Read this paper
  • Zhang H, Zhu L, Zuo W, et al. The ClC-3 chloride channel protein is a downstream target of cyclin D1 in nasopharyngeal carcinoma cells. Int J Biochem Cell Biol. 2013,45(3):672-83. doi: 10.1016/j.biocel.2012.12.015
    Read this paper

FAQ

Go to our frequently asked questions

Related products

IHC image of CSB-PA005074LA01HU diluted at 1:300 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Product group Antibodies
Cusabio
CDK6 AntibodyCSB-PA005074LA01HU
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetCDK6
  • SizePrice
Product group Antibodies
Nordic-MUbio
Mouse anti Human Cyclin Dependant Kinase 6C150M
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
TargetCDK6
  • SizePrice
Product group Antibodies
MedChem Express
CDK6 Antibody (YA511, Rabbit)HY-P80079
TargetCDK6
  • SizePrice
Figure 1. Western blot analysis of Cdk6 using anti-Cdk6 antibody (A00358). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cdk6 antigen affinity purified polyclonal antibody (Catalog # A00358) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cdk6 at approximately 37 kDa. The expected band size for Cdk6 is at 37 kDa.
Product group Antibodies
Boster Bio
Anti-Cdk6 Antibody Picoband(r)A00358-CARRIER-FREE
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetCDK6
  • SizePrice
Product group Antibodies
Antibodies.com
Anti-Cdk6 AntibodyA283461
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • SizePrice
Product group Antibodies
RayBiotech
Anti-CDK6 Antibody144-64306
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
TargetCDK6
  • SizePrice
Product group Antibodies
Bethyl Laboratories
Rabbit anti-CDK6 Antibody, Affinity PurifiedA304-937A
ApplicationsWestern Blot
TargetCDK6
  • SizePrice
Product group Antibodies
Applied Biological Materials
Anti-CDK6Y158361
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
Product group Antibodies
References
GeneTex
CDK6 antibody [N1C3]GTX103992
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetCDK6
  • SizePrice
Product group Antibodies
Assay Genie
CDK6 Monoclonal AntibodyCAB0106
TargetCDK6
  • SizePrice