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Paraformaldehyde-fixed, paraffin embedded human glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with CDK6 Polyclonal Antibody, Unconjugated (bs-0568R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with CDK6 Polyclonal Antibody, Unconjugated (bs-0568R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with CDK6 Polyclonal Antibody, Unconjugated (bs-0568R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.

CDK6 Polyclonal Antibody

BS-0568R
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCDK6
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    CDK6 Polyclonal Antibody
  • Delivery Days Customer
    7
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID1021
  • Target name
    CDK6
  • Target description
    cyclin dependent kinase 6
  • Target synonyms
    cell division protein kinase 6; cyclin-dependent kinase 6; MCPH12; PLSTIRE; serine/threonine-protein kinase PLSTIRE
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ00534
  • Protein Name
    Cyclin-dependent kinase 6
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Long non-coding RNA AK058003, as a precursor of miR-15a, interacts with HuR to inhibit the expression of gamma-synuclein in hepatocellular carcinoma cells. He X et al., 2017 Feb 7, Oncotarget
    Read more
  • 5-Aminolevulinic acid-mediated sonodynamic therapy induces anti-tumor effects in malignant melanoma via p53-miR-34a-Sirt1 axis. Hu Z et al., 2015 Aug, J Dermatol Sci
    Read more
  • miR-145 downregulates the expression of cyclin-dependent kinase 6 in human cervical carcinoma cells. Zhang J et al., 2014 Aug, Exp Ther Med
    Read more
  • The ClC-3 chloride channel protein is a downstream target of cyclin D1 in nasopharyngeal carcinoma cells. Zhang H et al., 2013 Mar, Int J Biochem Cell Biol
    Read more

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Figure 1. Western blot analysis of Cdk6 using anti-Cdk6 antibody (A00358). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cdk6 antigen affinity purified polyclonal antibody (Catalog # A00358) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cdk6 at approximately 37 kDa. The expected band size for Cdk6 is at 37 kDa.
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