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ProductsBack/CDKN1A Antibody
Western Blot analysis of COLO205 cells using p21 Polyclonal Antibody
Western Blot analysis of COLO205 cells using p21 Polyclonal Antibody
Western Blot analysis of COLO205 cells using p21 Polyclonal Antibody

CDKN1A Antibody

CSB-PA003623
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCDKN1A
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Overview

  • Supplier
    Cusabio
  • Product Name
    CDKN1A Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1026
  • Target name
    CDKN1A
  • Target description
    cyclin dependent kinase inhibitor 1A
  • Target synonyms
    CAP20; CDK-interacting protein 1; CDK-interaction protein 1; CDKN1; CIP1; cyclin-dependent kinase inhibitor 1; cyclin-dependent kinase inhibitor 1A (p21, Cip1); DNA synthesis inhibitor; MDA-6; melanoma differentiation associated protein 6; P21; p21CIP1; SDI1; WAF1; wild-type p53-activated fragment 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP38936
  • Protein Name
    Cyclin-dependent kinase inhibitor 1
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Immunofluorescence staining of A549 cells using the Anti-CDKN1A monoclonal antibody, showing specific staining in nucleoplasm in green. Microtubule probes are visualized in red (where available).
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Figure 1. Western blot analysis of P21/CDKN1A using anti-P21/CDKN1A antibody (A00145-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human U20S whole cell lysates, Lane 5: human SIHA whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P21/CDKN1A antigen affinity purified polyclonal antibody (Catalog # A00145-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for P21/CDKN1A at approximately 21 kDa. The expected band size for P21/CDKN1A is at 21 kDa.
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