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CEACAM1 Polyclonal Antibody

RD219308A
Reddot Biotech
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetCEACAM1
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Overview

  • Supplier
    Reddot Biotech
  • Product Name
    CEACAM1 Polyclonal Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.48 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID634
  • Target name
    CEACAM1
  • Target description
    CEA cell adhesion molecule 1
  • Target synonyms
    BGP, BGP1, BGPI, cell adhesion molecule CEACAM1, CD66a antigen, antigen CD66, carcinoembryonic antigen related cell adhesion molecule 1, carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP13688
  • Protein Name
    Cell adhesion molecule CEACAM1
  • Scientific Description
    This gene encodes a member of the carcinoembryonic antigen (CEA) gene family, which belongs to the immunoglobulin superfamily. Two subgroups of the CEA family, the CEA cell adhesion molecules and the pregnancy-specific glycoproteins, are located within a 1.2 Mb cluster on the long arm of chromosome 19. Eleven pseudogenes of the CEA cell adhesion molecule subgroup are also found in the cluster. The encoded protein was originally described in bile ducts of liver as biliary glycoprotein. Subsequently, it was found to be a cell-cell adhesion molecule detected on leukocytes, epithelia, and endothelia. The encoded protein mediates cell adhesion via homophilic as well as heterophilic binding to other proteins of the subgroup. - This is a CEACAM1 Polyclonal Antibody from Reddot Biotech. This product is for Research Use Only.
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CEACAM1 using anti-CEACAM1 antibody (A00923-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human HepG22 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEACAM1 antigen affinity purified polyclonal antibody (Catalog # A00923-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CEACAM1 at approximately 120-150 kDa. The expected band size for CEACAM1 is at 58 kDa.
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