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ProductsBack/CEBP alpha (Phospho-Thr226) Antibody HRP Conjugated

CEBP alpha (Phospho-Thr226) Antibody HRP Conjugated

MBS9461231
MyBioSource
Product group Antibodies
Price on request
0.1 ml
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Overview

  • Supplier
    MyBioSource
  • Product Name
    CEBP alpha (Phospho-Thr226) Antibody HRP Conjugated
  • Delivery Days Customer
    16
  • Certification
    Research Use Only
  • Concentration
    1 mg/ml
  • Conjugate
    HRP
  • Estimated Purity
    Purified by Protein A
  • Isotype
    IgG
  • UNSPSC
    12352203

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Figure 3. Flow Cytometry analysis of CACO-2 cells using anti-CEBP Alpha/CEBPA antibody (A00386-1). Overlay histogram showing CACO-2 cells stained with A00386-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CEBP Alpha/CEBPA Antibody (A00386-1, 1microg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10microg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1microg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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