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ProductsBack/C/EBP delta (C-6) Alexa Fluor(r) 647

C/EBP delta (C-6) Alexa Fluor(r) 647

SC-365546AF647
Santa Cruz Biotechnology
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetCEBPD
Price on request
200 ug
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Overview

  • Supplier
    Santa Cruz Biotechnology
  • Product Name
    C/EBP delta (C-6) Alexa Fluor(r) 647
  • Delivery Days Customer
    5
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Conjugate
    AlexaFluor 647
  • Gene ID1052
  • Target name
    CEBPD
  • Target description
    CCAAT enhancer binding protein delta
  • Target synonyms
    C/EBP-delta, CELF, CRP3, NF-IL6-beta, CCAAT/enhancer-binding protein delta, CCAAT/enhancer binding protein (C/EBP), delta, c/EBP delta, nuclear factor NF-IL6-beta
  • Host
    Mouse
  • Reactivity
    Human
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CEBP Delta/CEBPD using anti-CEBP Delta/CEBPD antibody (A03499-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human U87 whole cell lysates, Lane 4: mouse RAW264.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEBP Delta/CEBPD antigen affinity purified polyclonal antibody (Catalog # A03499-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CEBP Delta/CEBPD at approximately 36KD. The expected band size for CEBP Delta/CEBPD is at 36KD.
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