WB analysis of asynchronous and mitotically blocked HeLa lysates using GTX30232 CENPF antibody. Loading : 25microg
CENPF antibody
GTX30232
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityBovine, Human
TargetCENPF
Overview
- SupplierGeneTex
- Product NameCENPF antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1500 - 1:2500. ICC/IF: 1:200 - 1:1000. FACS: 1:50 - 1:200. IP: 1:50. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration2.5 mg/ml
- ConjugateUnconjugated
- FormulationLiquid
- Gene ID1063
- Target nameCENPF
- Target descriptioncentromere protein F
- Target synonymsAH antigen; cell-cycle-dependent 350K nuclear protein; CENF; CENP-F kinetochore protein; centromere protein F; centromere protein F, 350/400kDa; CILD31; hcp-1; kinetochore protein CENPF; mitosin; PRO1779; STROMS
- HostRabbit
- IsotypeIgG
- Protein IDP49454
- Protein NameCentromere protein F
- Scientific DescriptionThis gene encodes a protein that associates with the centromere-kinetochore complex. The protein is a component of the nuclear matrix during the G2 phase of interphase. In late G2 the protein associates with the kinetochore and maintains this association through early anaphase. It localizes to the spindle midzone and the intracellular bridge in late anaphase and telophase, respectively, and is thought to be subsequently degraded. The localization of this protein suggests that it may play a role in chromosome segregation during mitotis. It is thought to form either a homodimer or heterodimer. Autoantibodies against this protein have been found in patients with cancer or graft versus host disease. [provided by RefSeq]
- ReactivityBovine, Human
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203