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WB analysis of asynchronous and mitotically blocked HeLa lysates using GTX30232 CENPF antibody. Loading : 25microg
WB analysis of asynchronous and mitotically blocked HeLa lysates using GTX30232 CENPF antibody. Loading : 25microg
WB analysis of asynchronous and mitotically blocked HeLa lysates using GTX30232 CENPF antibody. Loading : 25microg

CENPF antibody

Research Use Only
GTX30232
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityBovine, Human
TargetCENPF
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Overview

  • Supplier
    GeneTex
  • Product Name
    CENPF antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:1500 - 1:2500. ICC/IF: 1:200 - 1:1000. FACS: 1:50 - 1:200. IP: 1:50. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    2.5 mg/ml
  • Conjugate
    Unconjugated
  • Formulation
    Liquid
  • Gene ID1063
  • Target name
    CENPF
  • Target description
    centromere protein F
  • Target synonyms
    AH antigen; cell-cycle-dependent 350K nuclear protein; CENF; CENP-F kinetochore protein; centromere protein F; centromere protein F, 350/400kDa; CILD31; hcp-1; kinetochore protein CENPF; mitosin; PRO1779; STROMS
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP49454
  • Protein Name
    Centromere protein F
  • Scientific Description
    This gene encodes a protein that associates with the centromere-kinetochore complex. The protein is a component of the nuclear matrix during the G2 phase of interphase. In late G2 the protein associates with the kinetochore and maintains this association through early anaphase. It localizes to the spindle midzone and the intracellular bridge in late anaphase and telophase, respectively, and is thought to be subsequently degraded. The localization of this protein suggests that it may play a role in chromosome segregation during mitotis. It is thought to form either a homodimer or heterodimer. Autoantibodies against this protein have been found in patients with cancer or graft versus host disease. [provided by RefSeq]
  • Reactivity
    Bovine, Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Phosphorylation of Astrin Regulates Its Kinetochore Function. Chung HJ et al., 2016 Aug 19, J Biol Chem
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  • The RAD51 paralogs ensure cellular protection against mitotic defects and aneuploidy. Rodrigue A et al., 2013 Jan 1, J Cell Sci
    Read more