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Western blot analysis of extracts of A-375 cells using CEP164 Polyclonal Antibody at dilution of 1:1000.
Western blot analysis of extracts of A-375 cells using CEP164 Polyclonal Antibody at dilution of 1:1000.
Western blot analysis of extracts of A-375 cells using CEP164 Polyclonal Antibody at dilution of 1:1000.

CEP164 Polyclonal Antibody

E-AB-63983
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    CEP164 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    This gene encodes a centrosomal protein involved in microtubule organization, DNA damage response, and chromosome segregation. The encoded protein is required for assembly of primary cilia and localizes to mature centrioles. Defects in this gene are a cause of nephronophthisis-related ciliopathies. Two transcript variants encoding different isoforms have been found for this gene.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CEP164 using anti-CEP164 antibody (A05971-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human SIHA whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEP164 antigen affinity purified polyclonal antibody (Catalog # A05971-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CEP164 at approximately 164 kDa. The expected band size for CEP164 is at 164 kDa.
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