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Western blot analysis of CEP55 Antibody (N-term) in MDA-MB231, T47D cell line lysates (35ug/lane). CEP55 (arrow) was detected using the purified Pab.
Western blot analysis of CEP55 Antibody (N-term) in MDA-MB231, T47D cell line lysates (35ug/lane). CEP55 (arrow) was detected using the purified Pab.
Western blot analysis of CEP55 Antibody (N-term) in MDA-MB231, T47D cell line lysates (35ug/lane). CEP55 (arrow) was detected using the purified Pab.

CEP55 (N-term) Rabbit Polyclonal Antibody

AP17941PU-N
OriGene
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetCEP55
Price on request
400 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    CEP55 (N-term) Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID55165
  • Target name
    CEP55
  • Target description
    centrosomal protein 55
  • Target synonyms
    C10orf3, CT111, MARCH, URCC6, centrosomal protein of 55 kDa, cancer/testis antigen 111, centrosomal protein 55kDa, up-regulated in colon cancer 6
  • Host
    Rabbit
  • Protein IDQ53EZ4
  • Protein Name
    Centrosomal protein of 55 kDa
  • Scientific Description
    CEP55 (N-term) rabbit polyclonal antibody
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CEP55 using anti-CEP55 antibody (A03954-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human Hacat whole cell lysates, Lane 3: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEP55 antigen affinity purified polyclonal antibody (A03954-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CEP55 at approximately 54 kDa. The expected band size for CEP55 is at 54 kDa.
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Product group Antibodies
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Mouse testis lysates probed with CEP55 Polyclonal Antibody, Unconjugated (bs-7742R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Product group Antibodies
References
Bioss
CEP55 Polyclonal AntibodyBS-7742R
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ReactivityCanine, Equine, Human, Mouse, Porcine, Rat, Sheep
TargetCEP55
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Western Blot analysis of HuvEc cells using CEP55 Polyclonal Antibody
Product group Antibodies
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CEP55 AntibodyCSB-PA001584
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TargetCEP55
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Product group Antibodies
MedChem Express
CEP55 Antibody (YA2797, Rabbit)HY-P83052
TargetCEP55
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LifeSpan BioSciences
CEP55 AntibodyLS-C401521
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TargetCEP55
  • SizePrice
Immunohistochemical staining of human testis shows cytoplasmic positivity in seminiferus ducts.
Product group Antibodies
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Anti-CEP55 AntibodyHPA023430
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TargetCEP55
  • SizePrice
Sample (30 ug of whole cell lysate) A: U87-MG 10% SDS PAGE GTX112190 diluted at 1:1000
Product group Antibodies
References
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CEP55 antibody [N2C3]GTX112190
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman
TargetCEP55
  • SizePrice