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Ceruloplasmin antibody

GTX28749
GeneTex
ApplicationsImmunoPrecipitation
DownloadDatasheet
Product group Antibodies
TargetCP
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Overview

  • Supplier
    GeneTex
  • Product Name
    Ceruloplasmin antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    Applications for immunohistochemistry have not been determined
  • Applications
    ImmunoPrecipitation
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1356
  • Target name
    CP
  • Target description
    ceruloplasmin
  • Target synonyms
    ceruloplasmin; ceruloplasmin (ferroxidase); CP-2
  • Host
    Goat
  • Isotype
    IgG
  • Protein IDP00450
  • Protein Name
    Ceruloplasmin
  • Scientific Description
    The protein encoded by this gene is a metalloprotein that binds most of the copper in plasma and is involved in the peroxidation of Fe(II)transferrin to Fe(III) transferrin. Mutations in this gene cause aceruloplasminemia, which results in iron accumulation and tissue damage, and is associated with diabetes and neurologic abnormalities. Two transcript variants, one protein-coding and the other not protein-coding, have been found for this gene. [provided by RefSeq, Feb 2012]
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Ceruloplasmin using anti-Ceruloplasmin antibody (PB9852). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: 22RV1 whole cell lysates, Lane 2: A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ceruloplasmin antigen affinity purified polyclonal antibody (Catalog # PB9852) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Ceruloplasmin at approximately 122 kDa. The expected band size for Ceruloplasmin is at 122 kDa.
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