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ProductsBack/cGKI (PRKG1) Mouse Monoclonal Antibody [Clone ID: OTI9G4]
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY PRKG1 (Cat# RC220986, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRKG1(Cat# TA501151). Positive lysates [LY416766] (100ug) and [LC416766] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY PRKG1 (Cat# RC220986, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRKG1(Cat# TA501151). Positive lysates [LY416766] (100ug) and [LC416766] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY PRKG1 (Cat# RC220986, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRKG1(Cat# TA501151). Positive lysates [LY416766] (100ug) and [LC416766] (20ug) can be purchased separately from OriGene.

cGKI (PRKG1) Mouse Monoclonal Antibody [Clone ID: OTI9G4]

CF501151
OriGene
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPRKG1
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    cGKI (PRKG1) Mouse Monoclonal Antibody [Clone ID: OTI9G4]
  • Delivery Days Customer
    14
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI9G4
  • Gene ID5592
  • Target name
    PRKG1
  • Target description
    protein kinase cGMP-dependent 1
  • Target synonyms
    AAT8, PKG, PKG1, PRKG1B, PRKGR1B, cGK, cGK 1, cGK1, cGKI, cGKI-BETA, cGKI-alpha, cGMP-dependent protein kinase 1, protein kinase, cGMP-dependent, regulatory, type I, beta, protein kinase, cGMP-dependent, type I
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ13976
  • Protein Name
    cGMP-dependent protein kinase 1
  • Scientific Description
    Carrier-free (BSA/glycerol-free) PRKG1 mouse monoclonal antibody, clone OTI9G4 (formerly 9G4)
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of CGKI/PRKG1 using anti-CGKI/PRKG1 antibody (A01708-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat lung tissue lysates, Lane 2: rat heart tissue lysates, Lane 3: mouse lung tissue lysates, Lane 4: human HEK293 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CGKI/PRKG1 antigen affinity purified polyclonal antibody (Catalog # A01708-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CGKI/PRKG1 at approximately 78KD. The expected band size for CGKI/PRKG1 is at 78KD.
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