More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/CHD4 antibody

CHD4 antibody

GTX124186
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetCHD4
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    GeneTex
  • Product Name
    CHD4 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID1108
  • Target name
    CHD4
  • Target description
    chromodomain helicase DNA binding protein 4
  • Target synonyms
    ATP-dependent helicase CHD4; CHD-4; chromodomain-helicase-DNA-binding protein 4; Mi-2 autoantigen 218 kDa protein; Mi-2b; Mi2-BETA; SIHIWES
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14839
  • Protein Name
    Chromodomain-helicase-DNA-binding protein 4
  • Scientific Description
    The product of this gene belongs to the SNF2/RAD54 helicase family. It represents the main component of the nucleosome remodeling and deacetylase complex and plays an important role in epigenetic transcriptional repression. Patients with dermatomyositis develop antibodies against this protein. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • CHD4 as an important mediator in regulating the malignant behaviors of colorectal cancer. Chang CL et al., 2021, Int J Biol Sci
    Read more
  • Identification of CHD4-beta1 integrin axis as a prognostic marker in triple-negative breast cancer using next-generation sequencing and bioinformatics. Ou-Yang F et al., 2019 Dec 1, Life Sci
    Read more
  • Over-Expression of CHD4 Is an Independent Biomarker of Poor Prognosis in Patients with Rectal Cancers Receiving Concurrent Chemoradiotherapy. Wang HC et al., 2019 Aug 21, Int J Mol Sci
    Read more
  • CHD4-mediated loss of E-cadherin determines metastatic ability in triple-negative breast cancer cells. Luo CW et al., 2018 Feb 1, Exp Cell Res
    Read more
  • The NuRD complex-mediated p21 suppression facilitates chemoresistance in BRCA-proficient breast cancer. Hou MF et al., 2017 Oct 15, Exp Cell Res
    Read more
  • Senataxin controls meiotic silencing through ATR activation and chromatin remodeling. Yeo AJ et al., 2015, Cell Discov
    Read more

Related products

Product group Antibodies
GeneTex
CHD4 antibody [HL1798]GTX637450
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetCHD4
  • SizePrice
WB analysis of various samples using GTX66800 CHD4 antibody. Lane1 : Hela whole cell lysate(40microg) Lane2 : NIH-3T3 whole cell lysate(40microg) Lane3 : H9C2 whole cell lysate(40microg) Lane4 : PC12 whole cell lysate(40microg) Dilution : 1:500
Product group Antibodies
GeneTex
CHD4 antibodyGTX66800
ApplicationsWestern Blot
TargetCHD4
  • SizePrice
Product group Antibodies
MedChem Express
CHD4 Antibody (YA946)HY-P81265
TargetCHD4
  • SizePrice
Product group Antibodies
GeneTex
CHD4 antibodyGTX101662
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetCHD4
  • SizePrice
WB analysis of indicated lysates using CHD4 antibody at a dilution of 1:1,000.
Product group Antibodies
GeneTex
CHD4 antibody [5F1-A11-C9]GTX49139
ApplicationsWestern Blot
TargetCHD4
  • SizePrice
Immunohistochemical staining of human endometrium shows strong nuclear positivity in glandular cells.
Product group Antibodies
Atlas Antibodies
Anti-CHD4 AntibodyHPA012008
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry, Chip-exoSeq
TargetCHD4
  • SizePrice
Product group Antibodies
Cusabio
CHD4 AntibodyCSB-PA006765
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
TargetCHD4
  • SizePrice
Figure 1. Western blot analysis of CHD4 using anti-CHD4 antibody (A01125-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U20S whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CHD4 antigen affinity purified polyclonal antibody (Catalog # A01125-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CHD4 at approximately 260 kDa. The expected band size for CHD4 is at 218 kDa.
Product group Antibodies
Boster Bio
Anti-CHD4 Antibody Picoband(r)A01125-3-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot
TargetCHD4
  • SizePrice
Anti-CHD4 Antibody
Product group Antibodies
Antibodies.com
Anti-CHD4 AntibodyA98218
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
  • SizePrice
Product group Antibodies
RayBiotech
Anti-CHD4 Antibody144-11574
ApplicationsWestern Blot
TargetCHD4
  • SizePrice