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ProductsBack/CHEK1 / CHK1 Antibody

CHEK1 / CHK1 Antibody

LS-C812425
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetCHEK1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CHEK1 / CHK1 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA (1:10000), WB (1:500 - 1:2000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID1111
  • Target name
    CHEK1
  • Target description
    checkpoint kinase 1
  • Target synonyms
    cell cycle checkpoint kinase; Checkpoint, S. pombe, homolog of, 1; CHK1; CHK1 checkpoint homolog; Chk1-S; serine/threonine-protein kinase Chk1
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Chk1/CHEK1 using anti-Chk1/CHEK1 antibody (A01060). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Chk1/CHEK1 antigen affinity purified polyclonal antibody (Catalog # A01060) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Chk1/CHEK1 at approximately 54 kDa. The expected band size for Chk1/CHEK1 is at 54 kDa.
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