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Formalin-fixed and paraffin embedded human cervical carcinoma labeled with Anti-CGA/ Chromogranin A Polyclonal Antibody, Unconjugated (bs-0539R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human cervical carcinoma labeled with Anti-CGA/ Chromogranin A Polyclonal Antibody, Unconjugated (bs-0539R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human cervical carcinoma labeled with Anti-CGA/ Chromogranin A Polyclonal Antibody, Unconjugated (bs-0539R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

CHGA Polyclonal Antibody

BS-0539R
Bioss
ApplicationsImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCHGA
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Overview

  • Supplier
    Bioss
  • Product Name
    CHGA Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID1113
  • Target name
    CHGA
  • Target description
    chromogranin A
  • Target synonyms
    CGA, PHE5, PHES, chromogranin-A, SP-I, betagranin (N-terminal fragment of chromogranin A), catestatin, chromofungin, parathyroid secretory protein 1, pituitary secretory protein I
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP10645
  • Protein Name
    Chromogranin-A
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Liu Y, Nelson T, Cromeens B, et al. HB-EGF embedded in PGA/PLLA scaffolds via subcritical CO2 augments the production of tissue engineered intestine. Biomaterials. 2016,103:150-159. doi: 10.1016/j.biomaterials.2016.06.039
    Read this paper
  • Liu XQ, Zhang HF, Zhang WD, et al. Regulation of neuroendocrine cells and neuron factors in the ovary by zinc oxide nanoparticles. Toxicol Lett. 2016,256:19-32. doi: 10.1016/j.toxlet.2016.05.007
    Read this paper
  • Wang Y, Ahmad AA, Sims CE, et al. In vitro generation of colonic epithelium from primary cells guided by microstructures. Lab Chip. 2014,14(9):1622-31. doi: 10.1039/c3lc51353j
    Read this paper
  • Wang Y, Ahmad AA, Shah PK, et al. Capture and 3D culture of colonic crypts and colonoids in a microarray platform. Lab Chip. 2013,13(23):4625-34. doi: 10.1039/c3lc50813g
    Read this paper

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Figure 1. Western blot analysis of Chromogranin A/CHGA using anti-Chromogranin A/CHGA antibody (A01256-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Chromogranin A/CHGA antigen affinity purified polyclonal antibody (Catalog # A01256-4) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Chromogranin A/CHGA at approximately 70 kDa. The expected band size for Chromogranin A/CHGA is at 51 kDa.
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