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Western blot analysis of extracts of various cell lines using CHIA Polyclonal Antibody at dilution of 1:1000.
Western blot analysis of extracts of various cell lines using CHIA Polyclonal Antibody at dilution of 1:1000.
Western blot analysis of extracts of various cell lines using CHIA Polyclonal Antibody at dilution of 1:1000.

CHIA Polyclonal Antibody

E-AB-65437
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    CHIA Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    The protein encoded by this gene degrades chitin, which is found in the cell wall of most fungi as well as in arthropods and some nematodes. The encoded protein can also stimulate interleukin 13 expression, and variations in this gene can lead to asthma susceptibility. Several transcript variants encoding a few different isoforms have been found for this gene.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of AMCase/CHIA using anti-AMCase/CHIA antibody (A00437-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat stomach tissue lysates, Lane 2: mouse stomach tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AMCase/CHIA antigen affinity purified polyclonal antibody (Catalog # A00437-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AMCase/CHIA at approximately 55 kDa. The expected band size for AMCase/CHIA is at 55 kDa.
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