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WB analysis of various sample lysates using GTX65611 CISD1 antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX65611 CISD1 antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX65611 CISD1 antibody. Dilution : 1:1000 Loading : 25microg per lane

CISD1 antibody

GTX65611
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetCISD1
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Overview

  • Supplier
    GeneTex
  • Product Name
    CISD1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:1000 - 1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID55847
  • Target name
    CISD1
  • Target description
    CDGSH iron sulfur domain 1
  • Target synonyms
    C10orf70, MDS029, ZCD1, mitoNEET, CDGSH iron-sulfur domain-containing protein 1, cysteine transaminase CISD1, zinc finger CDGSH-type domain 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NZ45
  • Protein Name
    CDGSH iron-sulfur domain-containing protein 1
  • Scientific Description
    This gene encodes a protein with a CDGSH iron-sulfur domain and has been shown to bind a redox-active [2Fe-2S] cluster. The encoded protein has been localized to the outer membrane of mitochondria and is thought to play a role in regulation of oxidation. Genes encoding similar proteins are located on chromosomes 4 and 17, and a pseudogene of this gene is located on chromosome 2. [provided by RefSeq, Feb 2012]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MitoNEET/CISD1 using anti-MitoNEET/CISD1 antibody (A04360-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human SW620 whole cell lysates, Lane 2: monkey heart tissue lysates, Lane 3: rat heart tissue lysates, Lane 4: rat kidney tissue lysates, Lane 5: mouse heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MitoNEET/CISD1 antigen affinity purified polyclonal antibody (Catalog # A04360-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MitoNEET/CISD1 at approximately 15KD. The expected band size for MitoNEET/CISD1 is at 12KD.
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