Unboiled various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Claudin 5 antibody [HL3156] (GTX640666) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
![Unboiled mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Claudin 5 antibody [HL3156] (GTX640666) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX640666/GTX640666_T-45481_20240802_WB_M_lung_24080602_667.webp "Unboiled mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Claudin 5 antibody [HL3156] (GTX640666) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Claudin 5 antibody [HL3156] detects Claudin 5 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. Claudin 5 stained by Claudin 5 antibody [HL3156] (GTX640666) diluted at 1:2000. Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX640666/GTX640666_45537_20250829_IHC-P_M_Multiple_2_25090420_536.webp "Claudin 5 antibody [HL3156] detects Claudin 5 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. Claudin 5 stained by Claudin 5 antibody [HL3156] (GTX640666) diluted at 1:2000. Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min")
![Claudin 5 antibody [HL3156] detects Claudin 5 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Green: Claudin 5 stained by Claudin 5 antibody [HL3156] (GTX640666) diluted at 1:2000. Red: beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX640666/GTX640666_45537_20250829_IHC-P_M_3_25090420_780.webp "Claudin 5 antibody [HL3156] detects Claudin 5 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Green: Claudin 5 stained by Claudin 5 antibody [HL3156] (GTX640666) diluted at 1:2000. Red: beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min")
![Claudin 5 antibody [HL3156] detects Claudin 5 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. Claudin 5 stained by Claudin 5 antibody [HL3156] (GTX640666) diluted at 1:2000. Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX640666/GTX640666_45537_20250829_IHC-P_M_Multiple_1_25090420_210.webp "Claudin 5 antibody [HL3156] detects Claudin 5 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. Claudin 5 stained by Claudin 5 antibody [HL3156] (GTX640666) diluted at 1:2000. Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min")
![Claudin 5 antibody [HL3156] detects Claudin 5 protein by immunohistochemical analysis. Sample: Paraffin-embedded human kidney. Claudin 5 stained by Claudin 5 antibody [HL3156] (GTX640666) diluted at 1:3200. Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX640666/GTX640666_45537_20250919_IHC-P_25092519_372.webp "Claudin 5 antibody [HL3156] detects Claudin 5 protein by immunohistochemical analysis. Sample: Paraffin-embedded human kidney. Claudin 5 stained by Claudin 5 antibody [HL3156] (GTX640666) diluted at 1:3200. Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min")
![Claudin 5 antibody [HL3156] detects Claudin 5 protein by immunohistochemical analysis. Sample: Paraffin-embedded human tissues. Claudin 5 stained by Claudin 5 antibody [HL3156] (GTX640666) diluted at 1:3200. Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX640666/GTX640666_45537_20250919_IHC-P_Multiple_RPKM_1_25092519_569.webp "Claudin 5 antibody [HL3156] detects Claudin 5 protein by immunohistochemical analysis. Sample: Paraffin-embedded human tissues. Claudin 5 stained by Claudin 5 antibody [HL3156] (GTX640666) diluted at 1:3200. Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min")
![Claudin 5 antibody [HL3156] detects Claudin 5 protein by immunohistochemical analysis. Sample: Paraffin-embedded human tissues. Claudin 5 stained by Claudin 5 antibody [HL3156] (GTX640666) diluted at 1:3200. Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX640666/GTX640666_45537_20250919_IHC-P_Multiple_RPKM_2_25092520_346.webp "Claudin 5 antibody [HL3156] detects Claudin 5 protein by immunohistochemical analysis. Sample: Paraffin-embedded human tissues. Claudin 5 stained by Claudin 5 antibody [HL3156] (GTX640666) diluted at 1:3200. Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min")
![Claudin 5 antibody [HL3156] detects Claudin 5 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat cerebellum. Claudin 5 stained by Claudin 5 antibody [HL3156] (GTX640666) diluted at 1:2000. Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX640666/GTX640666_45537_20250926_IHC-P_R_2_25100122_555.webp "Claudin 5 antibody [HL3156] detects Claudin 5 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat cerebellum. Claudin 5 stained by Claudin 5 antibody [HL3156] (GTX640666) diluted at 1:2000. Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min")
![Claudin 5 antibody [HL3156] detects Claudin 5 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat kidney. Claudin 5 stained by Claudin 5 antibody [HL3156] (GTX640666) diluted at 1:2000. Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX640666/GTX640666_45537_20250926_IHC-P_R_1_25100122_565.webp "Claudin 5 antibody [HL3156] detects Claudin 5 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat kidney. Claudin 5 stained by Claudin 5 antibody [HL3156] (GTX640666) diluted at 1:2000. Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min")
Unboiled various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Claudin 5 antibody [HL3156] (GTX640666) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Claudin 5 antibody [HL3156]
GTX640666
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse
TargetCLDN5
Overview
- SupplierGeneTex
- Product NameClaudin 5 antibody [HL3156]
- Delivery Days Customer7
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL3156
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID7122
- Target nameCLDN5
- Target descriptionclaudin 5
- Target synonymsAWAL, BEC1, CPETRL1, TMDVCF, TMVCF, claudin-5, transmembrane protein deleted in VCFS, transmembrane protein deleted in velocardiofacial syndrome
- HostRabbit
- IsotypeIgG
- Protein IDO00501
- Protein NameClaudin-5
- Scientific DescriptionThis gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets. Mutations in this gene have been found in patients with velocardiofacial syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, May 2018]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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