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Western Blot analysis of A549 cells using Claudin-5 Polyclonal Antibody
Western Blot analysis of A549 cells using Claudin-5 Polyclonal Antibody
Western Blot analysis of A549 cells using Claudin-5 Polyclonal Antibody

CLDN5 Antibody

CSB-PA001672
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCLDN5
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Overview

  • Supplier
    Cusabio
  • Product Name
    CLDN5 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7122
  • Target name
    CLDN5
  • Target description
    claudin 5
  • Target synonyms
    AWAL, BEC1, CPETRL1, TMDVCF, TMVCF, claudin-5, transmembrane protein deleted in VCFS, transmembrane protein deleted in velocardiofacial syndrome
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO00501
  • Protein Name
    Claudin-5
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Unboiled various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Claudin 5 antibody [HL3156] (GTX640666) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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Figure 1. IHC analysis of Claudin 5/CLDN5 using anti-Claudin 5/CLDN5 antibody (A03260-3). Claudin 5/CLDN5 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2microg/ml rabbit anti-Claudin 5/CLDN5 Antibody (A03260-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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