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ProductsBack/CLEC7A / Dectin 1 Antibody (clone 2A11)

CLEC7A / Dectin 1 Antibody (clone 2A11)

LS-C188265
LifeSpan BioSciences
ApplicationsFlow Cytometry, ImmunoPrecipitation, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
Product group Antibodies
TargetCLEC7A
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CLEC7A / Dectin 1 Antibody (clone 2A11)
  • Delivery Days Customer
    23
  • Application Supplier Note
    The epitope recognized by 2A11 is sensitive to H202 treatment, therefore quenching with 0.3% H202 should be performed after incubation with the primary antibody. The epitope recognized by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Acetone fixation should be used for frozen sections.. Flo (1:10 - 1:50), IHC-Fr, IP The epitope recognized by 2A11 is sensitive to H202 treatment, therefore quenching with 0.3% H202 should be performed after incubation with the primary antibody. The epitope recognized by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Acetone fixation should be used for frozen sections. IHC-P, IHC-R
  • Applications
    Flow Cytometry, ImmunoPrecipitation, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    2A11
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID64581
  • Target name
    CLEC7A
  • Target description
    C-type lectin domain containing 7A
  • Target synonyms
    beta-glucan receptor; BGR; CANDF4; CD369; CLECSF12; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 12; C-type lectin domain family 7 member A; C-type lectin superfamily member 12; DC-associated C-type lectin 1; DECTIN1; dectin-1; dendritic cell-associated C-type lectin-1; lectin-like receptor 1; SCARE2
  • Host
    Rat
  • Isotype
    IgG2b
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Dectin-1/CLEC7A using anti-Dectin-1/CLEC7A antibody (A02731-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Dectin-1/CLEC7A antigen affinity purified polyclonal antibody (Catalog # A02731-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Dectin-1/CLEC7A at approximately 36 kDa. The expected band size for Dectin-1/CLEC7A is at 28 kDa.
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