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ProductsBack/CLEC9A Antibody (Preservative Free)

CLEC9A Antibody (Preservative Free)

LS-C815576
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetCLEC9A
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CLEC9A Antibody (Preservative Free)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID283420
  • Target name
    CLEC9A
  • Target description
    C-type lectin domain containing 9A
  • Target synonyms
    CD370; C-type lectin domain family 9 member A; DNGR1; DNGR-1; HEEE9341; UNQ9341
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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The Binding Activity of Human CLEC9A with Anti-CLEC9A recombinant antibody Activity: Measured by its binding ability in a functional ELISA. Immobilized Anti-CLEC9A recombinant antibody at 2 microg/mL can bind Human CLEC9A protein (CSB-MP740922HU-B). The EC50 is 2.915-3.520 ng/mL.
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Figure 1. Flow Cytometry analysis of Jurkat cells using anti-CLEC9A antibody (A04577). Overlay histogram showing Jurkat cells stained with A04577 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CLEC9A Antibody (A04577, 1microg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10microg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1microg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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