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ProductsBack/CLIP1 / CLIP-170 Antibody

CLIP1 / CLIP-170 Antibody

LS-C747597
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetCLIP1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CLIP1 / CLIP-170 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 156kDa/160kDa/162kDa, while the observed MW by Western blot was 162kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) The predicted MW is 156kDa/160kDa/162kDa, while the observed MW by Western blot was 162kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID6249
  • Target name
    CLIP1
  • Target description
    CAP-Gly domain containing linker protein 1
  • Target synonyms
    CLIP, CLIP-170, CLIP170, CYLN1, RSN, CAP-Gly domain-containing linker protein 1, cytoplasmic linker protein 1, cytoplasmic linker protein 170 alpha-2, cytoplasmic linker protein CLIP-170, restin (Reed-Steinberg cell-expressed intermediate filament-associated protein)
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CLIP170 using anti-CLIP170 antibody (M08907). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human SiHa whole cell lysates, Lane 3: human A549 whoel cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CLIP170 antigen affinity purified monoclonal antibody (M08907) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CLIP170 at approximately 170 kDa. The expected band size for CLIP170 is at 162 kDa.
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