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ProductsBack/CLIP170 antibody [N1], N-term

CLIP170 antibody [N1], N-term

GTX117504
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetCLIP1
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Overview

  • Supplier
    GeneTex
  • Product Name
    CLIP170 antibody [N1], N-term
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6249
  • Target name
    CLIP1
  • Target description
    CAP-Gly domain containing linker protein 1
  • Target synonyms
    CLIP, CLIP-170, CLIP170, CYLN1, RSN, CAP-Gly domain-containing linker protein 1, cytoplasmic linker protein 1, cytoplasmic linker protein 170 alpha-2, cytoplasmic linker protein CLIP-170, restin (Reed-Steinberg cell-expressed intermediate filament-associated protein)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP30622
  • Protein Name
    CAP-Gly domain-containing linker protein 1
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Barbiero I, Peroni D, Siniscalchi P, et al. Pregnenolone and pregnenolone-methyl-ether rescue neuronal defects caused by dysfunctional CLIP170 in a neuronal model of CDKL5 Deficiency Disorder. Neuropharmacology. 2020,164:107897. doi: 10.1016/j.neuropharm.2019.107897
    Read this paper
  • Barbiero I, Peroni D, Tramarin M, et al. The neurosteroid pregnenolone reverts microtubule derangement induced by the loss of a functional CDKL5-IQGAP1 complex. Hum Mol Genet. 2017,26(18):3520-3530. doi: 10.1093/hmg/ddx237
    Read this paper

Datasheet

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Figure 1. Western blot analysis of CLIP170 using anti-CLIP170 antibody (M08907). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human SiHa whole cell lysates, Lane 3: human A549 whoel cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CLIP170 antigen affinity purified monoclonal antibody (M08907) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CLIP170 at approximately 170 kDa. The expected band size for CLIP170 is at 162 kDa.
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