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ProductsBack/CLPP Mouse Monoclonal Antibody [Clone ID: OTI1E2]
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CLPP (RC200301, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CLPP. Positive lysates LY416925 (100ug) and LC416925 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CLPP (RC200301, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CLPP. Positive lysates LY416925 (100ug) and LC416925 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CLPP (RC200301, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CLPP. Positive lysates LY416925 (100ug) and LC416925 (20ug) can be purchased separately from OriGene.

CLPP Mouse Monoclonal Antibody [Clone ID: OTI1E2]

CF502065
OriGene
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityCanine, Human, Monkey, Mouse, Rat
TargetCLPP
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    CLPP Mouse Monoclonal Antibody [Clone ID: OTI1E2]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI1E2
  • Gene ID8192
  • Target name
    CLPP
  • Target description
    caseinolytic mitochondrial matrix peptidase proteolytic subunit
  • Target synonyms
    DFNB81, PRLTS3, ATP-dependent Clp protease proteolytic subunit, mitochondrial, ATP-dependent protease ClpAP, proteolytic subunit, human, ClpP caseinolytic peptidase ATP-dependent, proteolytic subunit, ClpP caseinolytic peptidase, ATP-dependent, proteolytic subunit homolog, ClpP caseinolytic protease, ATP-dependent, proteolytic subunit homolog, endopeptidase Clp, putative ATP-dependent Clp protease proteolytic subunit, mitochondrial
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ16740
  • Protein Name
    ATP-dependent Clp protease proteolytic subunit, mitochondrial
  • Scientific Description
    Carrier-free (BSA/glycerol-free) CLPP mouse monoclonal antibody, clone OTI1E2 (formerly 1E2)
  • Reactivity
    Canine, Human, Monkey, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of CLPP using anti-CLPP antibody (A01117-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human THP-1 whole cell lysates, Lane 5: rat pancreas tissue lysates, Lane 6: rat lung tissue lysates, Lane 7: mouse pancreas tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CLPP antigen affinity purified polyclonal antibody (Catalog # A01117-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CLPP at approximately 26KD. The expected band size for CLPP is at 26KD.
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