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WB analysis of various sample lysates using GTX64834 CMG1 antibody. Dilution : 1:3000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX64834 CMG1 antibody. Dilution : 1:3000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX64834 CMG1 antibody. Dilution : 1:3000 Loading : 25microg per lane

CMG1 antibody

GTX64834
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetIFT74
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Overview

  • Supplier
    GeneTex
  • Product Name
    CMG1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:1000 - 1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID80173
  • Target name
    IFT74
  • Target description
    intraflagellar transport 74
  • Target synonyms
    BBS22; capillary morphogenesis gene 1 protein; capillary morphogenesis protein 1; CCDC2; CMG1; CMG-1; coiled-coil domain containing 2; coiled-coil domain-containing protein 2; intraflagellar transport 74 homolog; intraflagellar transport protein 74 homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96LB3
  • Protein Name
    Intraflagellar transport protein 74 homolog
  • Scientific Description
    This gene encodes a core intraflagellar transport (IFT) protein which belongs to a multi-protein complex involved in the transport of ciliary proteins along axonemal microtubules. IFT proteins are found at the base of the cilium as well as inside the cilium, where they assemble into long arrays between the ciliary base and tip. This protein, together with intraflagellar transport protein 81, binds and transports tubulin within cilia and is required for ciliogenesis. Naturally occurring mutations in this gene are associated with amyotrophic lateral sclerosis--frontotemporal dementia and Bardet-Biedl Syndrome. [provided by RefSeq, Mar 2017]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IFT74 using anti-IFT74 antibody (A08931-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human Raji whole cell lysates, Lane 4: monkey COS-7 whole cell lysates, Lane 5: rat thymus tissue lysates, Lane 6: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IFT74 antigen affinity purified polyclonal antibody (Catalog # A08931-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IFT74 at approximately 69 kDa. The expected band size for IFT74 is at 69 kDa.
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