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Western Blot analysis of Human fetal kidney and fetal muscle tissue using CNN3 Polyclonal Antibody at dilution of 1:400
Western Blot analysis of Human fetal kidney and fetal muscle tissue using CNN3 Polyclonal Antibody at dilution of 1:400
Western Blot analysis of Human fetal kidney and fetal muscle tissue using CNN3 Polyclonal Antibody at dilution of 1:400

CNN3 Polyclonal Antibody

E-AB-10711
Elabscience
ApplicationsWestern Blot
Product group Antibodies
TargetCNN3
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Overview

  • Supplier
    Elabscience
  • Product Name
    CNN3 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot
  • Applications Supplier
    ELISA WB IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.3 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID1266
  • Target name
    CNN3
  • Target description
    calponin 3
  • Target synonyms
    calponin-3, calponin 3, acidic, dJ639P13.2.2 (acidic calponin 3)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15417
  • Protein Name
    Calponin-3
  • Scientific Description
    This gene encodes a protein with a markedly acidic C terminus; the basic N-terminus is highly homologous to the N-terminus of a related gene, CNN1. Members of the CNN gene family all contain similar tandemly repeated motifs. This encoded protein is associated with the cytoskeleton but is not involved in contraction. Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

MSDS

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Figure 1. Western blot analysis of Calponin 3/CNN3 using anti-Calponin 3/CNN3 antibody (A08267-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat PC-12 whole cell lysates, Lane 3: human U20S whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Calponin 3/CNN3 antigen affinity purified polyclonal antibody (Catalog # A08267-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Calponin 3/CNN3 at approximately 36 kDa. The expected band size for Calponin 3/CNN3 is at 36 kDa.
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